A stochastic model to estimate worm burdens and associated risk factors in sheep naturally infected with Haemonchus contortus
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چکیده
Risk can be defined as the possibility of loss or injury coupled to the probability of such loss or injury (Singh 2000), and the main components of risk analysis are risk assessment, risk management and risk communication (Thrusfield 2001). Quantitative risk assessment is defined as a mathematical model containing inputs and outputs which are expressed numerically (Murray 2004). The work in the present chapter is concerned with the risk assessment part of risk analysis, as it is concerned with hazard identification, and estimation of the probability and magnitude of the risk of haemonchosis as indicated by simulating worm counts in sheep infected with H. contortus.
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A stochastic model accommodating the FAMACHA© system for estimating worm burdens and associated risk factors in sheep naturally infected with Haemonchus contortus.
A previously developed multiple regression algorithm was used as the basis of a stochastic model to simulate worm burdens in sheep naturally infected with Haemonchus contortus over five consecutive Haemonchus seasons (November to January/February) on a farm in the summer rainfall region in South Africa, although only one season is discussed. The algorithm associates haemoglobin levels with worm...
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Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. On western blot analysis, it was demonstrated that thiol-purified antigen showed a s...
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